MEMS-BASED CHARACTERIZATION OF BREAST CANCER CELLS AND COLON CANCER CELLS

Authors:

Shobha Gupta ,

DOI NO:

https://doi.org/10.26782/jmcms.2022.07.00001

Keywords:

MEMS,capacitance,breast cancer cells,colon cancer cells,

Abstract

The study of electrical characteristics of cells based on their biophysical properties, and their relevance with their status, has been a very useful non-invasive tool for disease diagnosis and treatment. A MEMS device is modelled and simulated for characterizing the electrical behavior of a type of breast cancer cells and colon cancer cells. The sample of highly invasive breast cancer cells (Hs 578T) was compared with the HT-29 colon cancer cells in the frequency range of 1 to 13 GHz. It is found that the rate of change of capacitance of the given colon cancer cells is less than that of the given highly metastatic breast cancer cells. This shows the difference in electrical characteristics of cells with different cell types and could be a basis for discriminating cell types and related metastasis.

Refference:

I. Aidley D. J., Stanfield P. R., “Ions on the move”, in Ion Channels: Molecules in Action, Cambridge, UK, Cambridge University Press, 1996.
II. Allison Timothy M. and Landreh Michael, Ion mobility in Structural Biology, Comprehensive Analytical Chemistry, Vol.83, 2019, pp.161-195.
III. Asami K., “Dielectric spectra of biological cells and tissues simulated by three-dimensional finite difference method”, ICEBI, IFMBE Proceedings book series, Springer, Vol.17, 2007, pp. 98-101.
IV. Bhura Dhiraj Kumar, “3D Interdigitated Electrode Array (IDEA) Biosensor For Detection Of Serum Biomarker, M.S. Thesis, Electrical and Computer Engineering, Portland State University, USA, 2011.
V. Cone Clarence D., “Unified Theory on the Basic Mechanism of Normal Mitotic Control and Oncogenesis” in Transmembrane Potentials and Characteristics of Immune and Tumor Cells, 1st edition, Boca Raton, Florida, CRC Press, Taylor & Francis Group, 1985.
VI. Cone C. D., “Variation of the transmembrane potential level as a basic mechanism of mitosis control”, Oncology, International Journal of Cancer research and treatment, Vol. 24, Issue 6, 1970, pp. 438-470.
VII. Cone C. D., “The role of surface electrical transmembrane potential in normal and malignant mitogenesis”, Annals of New York Academy of Sciences, Vol. 238, pp. 420-435, 1974.
VIII. Cooper Geoffrey M., “Molecules and Membranes” & “The Plasma Membrane”, in The Cell: A Molecular Approach, Eighth edition, Sinauer Associates, Sunderland, New York, Oxford University Press, 2019.
IX. Cope Freeman W., “A medical application of the Ling Association-Induction Hypothesis: The high potassium, low sodium diet of the Gerson cancer therapy”, Physiological Chemistry and Physics, Vol.10, No.5, 1978, pg. 465-468.
X. Cope Freeman W., “A medical application of the Ling Association-Induction Hypothesis: The high potassium, low sodium diet of the Gerson cancer therapy”, Physiological Chemistry and Physics, Vol.10, No.5, pp. 465-468, 1978.
XI. Cure J. C., “Cancer an electrical phenomenon”, Resonant, Vol.1, No.1, 1991.
XII. Dean D.A., Ramanathanb T., Machadoa D., Sundararajana R., “Electrical impedance spectroscopy study of biological tissues”, Journal of Electrostatics, Vol.66, 2008, pg 165-177.
XIII. Dorey A. Robert, “Microstructure- property relationships”, in Ceramic Thick films for MEMS and Microdevices, First edition , Elsevier, USA, 2011.
XIV. Duan Wei, “Cell impedance of cancer cells: towards novel diagnostic and therapeutic selection methods”, M. Phil thesis, School of life sciences, University of Sussex, England, January 2013.
XV. Grimnes Sverre and Martinsen Orjan, “Passive Tissue Electrical Properties”, Bioimpedance and Bioelectricity Basics, 3rd edition, Academic Press, United States, 2014.
XVI. Guyton Arthur C. and Hall J.E., “Functional organization of Human Body and Control of the Internal Environment”, in Textbook of Medical Physiology, Twelfth Edition, Saunders, Elsevier, USA, 2011.
XVII. Huclova Sonja, “Modeling of Cell Suspensions and Biological Tissue for Computational Electromagnetics”, PhD Thesis in Chemistry, ETH Zurich, Switzerland, 2011.
XVIII. Mousa Hussein, Falah Awwad, Dwija Jithin, Husain El Hasasna, Khawlah Athamneh & Rabah Iratni, “Breast cancer cells exhibits specific dielectric signature in vitro using the open-ended coaxial probe technique from 200 MHz to 13.6 GHz”, Scientific Reports, Nature, Vol.9, Issue 1, 4681, March 2019.
XIX. Muhammad Asraf Mansor , Masaru Takeuchi , Masahiro Nakajima , Yasuhisa Hasegawa and Mohd Ridzuan Ahmad, “Electrical Impedance Spectroscopy for Detection of Cells in Suspensions Using Microfluidic Device with Integrated Microneedles”, Applied Sciences, Vol.7, issue 2, 170, February, 2017.
XX. Muhammad Asraf Mansor and Mohd Ridzuan Ahmad, “Single Cell Electrical Characterization Techniques”, International Journal of Molecular Sciences, Vol.16, 2015, pg. 12686-12712.
XXI. Nasir Nida and Ahmad Mahmoud Al, “Cells Electrical Characterization: Dielectric properties, Mixture and Modeling Theories”, Hindawi Journal of Engineering, Vol 2020, article id 9475490, 2020.
XXII. Qiao G, Duan W, Chatwin C, A Sinclair and W Wang, “Electrical Properties of Breast cancer cells from impedance measurement of cell suspensions, Journal of Physics, Vol 224(1), 012081, 2010.
XXIII. Revici Emanuel, “Biological Entities”, in Research in Pathophysiology as Basis for Guided Chemotherapy, with Special Application to Cancer, Princeton, New Jersey, New York, USA, D. Van Nostrand Company, 1961
XXIV. Schwan H.P. and Takashima Shiro, “Dielectric Behavior of Biological cells and Tissues”, Bulletin of the Institute for Chemical Research, Kyoto University, Vol.69, No.4, pg. 459-475, 1991.
XXV. Stern R. G., “Carcinogenesis and the plasma membrane”, Medical Hypotheses, Vol. 52, No.5, May 1999, pp. 367-372.

View Download