Identification of Genes in Soft Coral Scleronephthya Gracillimum Using Next Generation Sequencing

Authors:

Seonock Woo,Seungshic Yum,

DOI NO:

https://doi.org/10.26782/jmcms.2019.03.00036

Keywords:

Soft Coral ,Scleronephthya Gracillimum, Gene ,Cdna Chip ,Next Generation Sequencing,

Abstract

The soft coral Scleronephthya gracillimum is a colonial azooxanthellate coral being dominant in Seoguipo, Jeju Island, Korea (33°24'N, 126°32'E). This coral contributes to the survival and maintenance of the overall biological community in this area by offering a wide variety of habitats for benthic marine animals. Ultimately we aimed the construction of cDNA chip from this soft coral species for the health assessment using its transcriptional changes and we identified the soft coral genes using next generation sequencing (NGS) and searched their gene functions using BLAST algorithm and classified the functional categories based on KOG classification.

Refference:

I.Chu TY.(1974). The fluctuations of the Kuroshio current in the easternsea area of Taiwan.Acta Oceanogr Taiwan, 4: 1-12.

II.CostanzaR.Ralph d’Arge.,Rudolf de G., Stephen F.,Monica G.,Bruce H.,Karin L.,Shahid N.,Robert V. O’Neill.,Jose P.,Robert G. R.,Paul S.,and Marjan van den Belt.(1997). The value of the world’s cosystem services and natural capital. Nature,387:253-260.

III.Hsin YC., Wu CR., andShaw PT.(2008).Spatial and temporal variations of the Kuroshio east of Taiwan. 1982–2005: Anumeral studies. J Geophys Res, 113:C04002.

IV.Lu HJ.,andLee HL.(2014).Changes in the fish species composition in the coastal zones of the Kuroshio Current and China Coastal Current during periods of climate change: Observations from the set-net fishery (1993–2011). Fish Res, 155: 103-113.

V.Namias J. (1970).Macroscale variations in sea-surface temperatures in the North Pacific. J Geophys Res,75:565–582.

VI.Park WS.,andOh IS.(2000).Interannual and interdecadal variations of sea surface temperature in the East Asianmarginal Seas. Prog Oceanogr, 47:191-204.

VII.Purcell JE., Uye S.,andLo WT.(2007).Anthropogenic causes of jellyfish blooms and their direct consequences for humans:A review. MAR Ecol Prog Ser, 350:153-174.

VIII.Woo S., Yum S.,YoonM., KimSH., LeeJ,. KimJ H., andLee TK.(2005). Efficient isolation of intact RNA from the soft coral Scleronephthya gracillimum (Kükenthal) for gene expression analyses. Integr Biosci,9:205-209.

IX.Wu CR., Chang YL., Oey LY., Chang CWJ., andHsin YC.(2008).Air-sea interaction between tropical cyclone Nari andKuroshio. Geophys Lett, 35:L12605.

X.Yamaguchi M.(1986).Acanthaster planci infestations of reefs and coral assemblages in Japan:A retrospective analysis of control efforts. Coral Reefs, 5:23-30.

XI.Yamano H., Sugihara K.,andNomura K.(2011).Rapid poleward range expansion of tropical reef corals in response to rising sea surface temperatures. Geophys Res Lett,38: L04601.

XII.454 Life Sciences.(2003).Technology page. http://my454.com/products/technology.asp

View | Download