The Enhanced Extraction Conditions for Phenolic and Flavonoid Compounds from the Underutilized Red Pitaya Peels Using Response Surface Methodology

Authors:

Ramya Vijayakumar,Siti Salwa Abd Gani,Uswatun Hasanah Zaidan,

DOI NO:

https://doi.org/10.26782/jmcms.2019.03.00022

Keywords:

Response Surface Methodology,Red Pitaya Peels,Antioxidant ,TotalPhenolic Content,Total Flavonoid Content,

Abstract

In this study, response surface methodology (RSM) was augmented to determine the effects of independent variables, namely extraction temperature (45-65°C), ethanol concentration (70-90%) and extraction time (80-120 min) to optimize the retained antioxidant compounds of the red pitaya peels through responses which were total phenolic content (TPC) and total flavonoid content (TFC). Regression analysis showed that more than 90% of the variation was explained by the second-order polynomial models of the different responses and the experimental values displayed that the extraction conditions had significant effect (p<0.001) on the TPC and TFC respectively. The optimized conditions were ethanol 82% for 103 min at 56°C with values of 172.01 mg/g for TPC and 7.45 mg/g for TFC respectively, which were in a good agreement with those predicted, thus indicating the suitability of the employed model in optimizing the extraction conditions of the red pitaya peels and similar natural functional product optimization.

Refference:

I.Biesaga M., and Pyrzynska K. (2013). Stability of Bioactive Polyphenols from Honey during Different Extraction Methods. Food Chemistry, 136: 46-54.

II.Chan S.W., Wan Aida W.M., Lee C.Y., Yap C.F., and Ho C.W. (2009). Optimisation of extraction conditions for phenolic compounds from limau purut (Citrus hystrix) peels. Journal of International Food Research, 16: 203-213.

III.Davidov-Pardo G., Arozarena M.R.I., and Marin-Arroyo M.R. (2011). Stability of Polyphenolic Extracts from Grape Seeds after Thermal Treatments. European Food Research and Technology, 232: 211-220.

IV.He G.Q., Xiong H.P.,Chen Q.H., Ruan H., Wang Z.Y., and Traore L. (2005). Optimization of conditions for supercritical fluid extraction of flavonoids from hops (Humulus lupulus L.). Journal of Zhejiang University Science 6B, 10: 999-1004.

V.Hoa T.T., Clark C.J., Wadddell B.C., and Woolf A.B. (2006). Postharvest quality of dragon fuit

VI.(Hylocereus undatus) following disinfesting hot air treatments. Postharvest Biology and Technology, 41: 62-69.

VII.Kumar S.T., Baskar R., Shanmugam S., Rajsekaran P., Sadasivam S., andManikandan V. (2008). Optimization of flavonoids extraction from the leaves ofTabernaemontana heyneana Wall.using L16 Orthogonal design.Natural Science, 6: 14–25.

VIII.Liyana-Pathirana C.M., and Shahidi F. (2005). Optimization of extraction of phenoliccompounds from wheat using response surface methodology. Food Chemistry, 93: 47-56.

IX.Makris D.P., Boskou G., and Andrikopoulos N.K. (2007). Recovery of antioxidant phenolics from white vinification solid by-products employing water/ethanol mixtures. Bioresource Technology, 98: 2963−2967.

X.Naczk M., and Shahidi F. (2004). Extraction and analysis of phenolics in food. Journal of Chromatography A, 1054: 95-111.

XI.Nerd A., Sitrita Y., Kaushika R.A., and Mizrahi Y. 2002. High summer temperatures inhibit flowering in vine pitaya crops (Hylocereus spp). Scientia Horticulturae, 96: 343-350.

XII.Quettier-Deleu C., Gressier B., Vasseur J., Dine T., Brunet J., Luyck M., Cazin M., Cazin J.C., Bailleul F., and Trotin F. (2000). Phenolic compounds andantioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of Ethnopharmacolog, 72: 35-40.

XIII.Singleton V.L., Orthofer R., and Lamuela-Raventos R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152–178.

XIV.Spigno G., Tramelli L., and De Faveri D.M. (2007). Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. Journal of Food Engineering, 81: 200-208.

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